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Wednesday, January 30, 2013

Ahhhh!!

Hello, well let's see what is new to talk about.
First off, I did get a response back from the ASU Conference, so it looks like I will be presenting my project on March 4th. I am going to admit, I am somewhat scared and excited to have the opportunity to present at ASU. Let’s see how it goes.

As for my project I have hit a sort of wall. I realized that some of my test might have been misread and so I have decided to take the safe route and just redo some of the test in question.
What I found pretty interesting was the results of Strept. Mutans, which was one of the bacteria’s, in a glucose fermentation broth. Glucose FB is a broth that when inoculated with certain bacteria’s, will determine if an acid or gas (or both) is produced.
In the image to your right, you will see how the glucose broth originally looks like.
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If you can tell the broth is a sort of orange color and there is a small test tube in the larger test tube. Those are called duram test tubes and the purpose of those tests tubes is the read whether gas was produced from the bacteria. If the bacterium is positive for gas production then a bubble will appear on the top of the duram tube. The reading that I found interesting was of this image below.
  


If you can see there is a growth, or build up, in the duram tube where the gas bubble should be. This is something that I have never seen even with the other 14 bacteria’s that went through the same test. I did manage to ask several people about the growth but nobody knew what it could be. So what I then proceeded to do was basically redo the glucose test for Strept. Mutans. This will help me determine if this is usual for Strept. Mutans in glucose fermentation broths or if it could possibly be a contamination.





Ohh and just cause I want to share a little of my insanity, not really but almost there, I have uploaded another picture of the choas that surounds me currently. Thus the reason for the title Ahhh...







Wednesday, January 23, 2013

Update 01/23/2013


Well I am still working on my bacteria identification project. I am currently in the process of creating a dichotomous key that will make the identification of bacteria simpler, but I have also been able to determine what tests give the best and clearest results in the end. I have encountered a few bumps along the road but all in all I feel my dichotomous key is coming along just well. I am aiming to have a completed one by next week so I can have Matt edit it and I can move on from there. I have already entered my application for the MGE Conference at ASU, now all there is to do is wait for a response. The next step after the completion of the dichotomous key would be to begin on my poster. I have only done one other poster before this one but I am confident that I will be able to produce a more than excellent poster. I don’t have many pictures of what I am doing but I did take the liberty to take a picture of the mess I have created in the process of working.  

As messy as my area is beginning to look, I can only imagine how my brain is starting to look like =(