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Wednesday, January 30, 2013

Ahhhh!!

Hello, well let's see what is new to talk about.
First off, I did get a response back from the ASU Conference, so it looks like I will be presenting my project on March 4th. I am going to admit, I am somewhat scared and excited to have the opportunity to present at ASU. Let’s see how it goes.

As for my project I have hit a sort of wall. I realized that some of my test might have been misread and so I have decided to take the safe route and just redo some of the test in question.
What I found pretty interesting was the results of Strept. Mutans, which was one of the bacteria’s, in a glucose fermentation broth. Glucose FB is a broth that when inoculated with certain bacteria’s, will determine if an acid or gas (or both) is produced.
In the image to your right, you will see how the glucose broth originally looks like.
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If you can tell the broth is a sort of orange color and there is a small test tube in the larger test tube. Those are called duram test tubes and the purpose of those tests tubes is the read whether gas was produced from the bacteria. If the bacterium is positive for gas production then a bubble will appear on the top of the duram tube. The reading that I found interesting was of this image below.
  


If you can see there is a growth, or build up, in the duram tube where the gas bubble should be. This is something that I have never seen even with the other 14 bacteria’s that went through the same test. I did manage to ask several people about the growth but nobody knew what it could be. So what I then proceeded to do was basically redo the glucose test for Strept. Mutans. This will help me determine if this is usual for Strept. Mutans in glucose fermentation broths or if it could possibly be a contamination.





Ohh and just cause I want to share a little of my insanity, not really but almost there, I have uploaded another picture of the choas that surounds me currently. Thus the reason for the title Ahhh...







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